Improved separation of polymeric and monomeric protein fractions using a high resolution column

[摘要] The main objective of this study was to test the Yarra-SEC 4000 analytical column (300x 7.80 mm) against the BioSep-SEC s4000 narrow bore column (300 x 4.60 mm) thatwas used by most researchers up to now, for separation of wheat gluten proteins, usingSE-HPLC. The relationship between protein fractions and a range of qualitycharacteristics were then determined. The trials were conducted in the three productionareas of South Africa representing the dryland summer rainfall, dryland winter rainfalland irrigated regions. These regions each have specific cultivars developed for theirspecific conditions. The Yarra-SEC 4000 analytical column yielded nine profile peakscompared to the five of the BioSep-SEC s4000 narrow bore column. Highly significantdifferences were found between the protein fractions for the irrigated region and thewinter rainfall cultivars. Very few significant differences were seen for protein fractionsbetween cultivars in the dryland summer rainfall region. The GLI%p correlatedpositively with dough rheological characteristics in cultivars of the irrigated region. TheUPP positively correlated with dough rheological parameters for cultivars of the irrigatedregion. No significant correlations were observed between UPP and dough rheologicalcharacteristics in the dryland winter rainfall cultivars. The irrigated region cultivarspresented more significant correlations between protein fractions and qualitycharacteristics. Significant correlations were obtained between SDSVOL and proteinfractions for the winter rainfall region cultivars. No significant correlations were obtainedbetween SDSVOL and protein fractions for the irrigated and dryland summer rainfallcultivars. In this study it was observed that Yarra-SEC 4000 analytical column can beemployed successfully in the separation of wheat proteins.

[发布机构] University of the Free State