Factors contributing to sodium dodecyl sulphate (SDS) sedimentation in irrigation wheat

[摘要] The main objective of this study was to determine factors contributing to sodium dodecylsulphate-sedimentation volume (SDSVOL) in elite wheat breeding lines grown at threedifferent irrigation locations. This was possible through the use of sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE), mixograph analysis and sizeexclusion-high performance liquid chromatography (SE-HPLC) techniques. Genotypeand environment effects were highly significant for SDSVOL, measured qualitycharacteristics as well as the protein fractions, whereas genotype by environment (GxE)interaction was either significant or not significant for most of the measured variablesand highly significant for almost all the protein fractions except the absolute value.Relationships between SDSVOL and other quality characteristics varied in combinedand single location analysis of variance. The combined analysis shows a positiverelationship between SDSVOL and flour protein content (FPC), high molecular weightgluteninsubunits (HMW-GS) and dough characteristics. Protein fractions also revealedpositive relationships with SDSVOL and the measured characteristics such as FPC,envelope peak integral (EPI), midline right integral (MRI) and envelope peak time (EPT).Principal component analysis (PCA) reduced 21 quality characteristics to five principlecomponents, which explained 99.83% of total variance of envelope tail integral (ETI),midline left integral (MLI), midline peak integral (MPI), MRI, midline tail integral (MTI),SDSVOL and EPI. The SDSVOL and MRI showed the strongest discriminatory powercompared to the other measured quality characteristics. Five of the elite lines (13, 12, 7,2 and 1) can be recommended to breeders for further evaluation based on their goodquality characteristics and strong relationship with some measured qualitycharacteristics.

[发布机构] University of the Free State