Sodium butyrate inhibits angiogenesis of human intestinal microvascular endothelial cells through COX‐2 inhibition

[摘要]

We examined the effect of sodium butyrate on in vitro angiogenesis and cyclooxygenase (COX) expression using primary cultures of human intestinal microvascular endothelial cells (HIMEC). Butyrate inhibited VEGF-induced cellular proliferation, transmigration and tube formation of HIMEC. Butyrate also inhibited COX-2 expression as well as prostaglandin (PG)E₂ and PGI₂ production, and administration of PGI₂ analog partially reversed the effect of butyrate on HIMEC angiogenesis. These results indicate that sodium butyrate inhibits HIMEC angiogenesis through down-regulation of COX-2 expression and PG production, and suggest that anti-angiogenic mechanisms may also be involved in the inhibitory effect of sodium butyrate on tumor growth.

[学科分类] 生物化学/生物物理

[关键词] Human intestinal microvascular endothelial cell;Angiogenesis;Sodium butyrate;Cyclooxygenase-2;Prostaglandin I2;COX;cyclooxygenase;HIMEC;human intestinal microvascular endothelial cell;PG;prostaglandin;TX;thromboxane;SCFA;short-chain fatty acid;FBS;fetal bovine serum;VEGF;vascular endothelial growth factor;HRP;horseradish peroxydase;LPS;lipopolysaccharide;NSAID;non-steroidal anti-inflammatory drugs;eNOS;endothelial nitric oxide synthase