CD36‐mediated endocytic uptake of advanced glycation end products (AGE) in mouse 3T3‐L1 and human subcutaneous adipocytes

[摘要]

Interaction of advanced glycation end products (AGE) with AGE receptors induces several cellular phenomena potentially relating to diabetic complications. We here show that AGE-modified bovine serum albumin (BSA) is endocytosed by adipocytes via CD36. Upon differentiation, 3T3-L1 and human subcutaneous adipose cells showed marked increases in endocytic uptake and subsequent degradation of [¹²⁵I]AGE-BSA, which were inhibited effectively by the anti-CD36 antibody. Ligand specificity of CD36 for modified BSAs was compared with that of LOX-1 and scavenger receptor class A. Effect of fucoidan on [¹²⁵I]AGE-BSA binding showed a sharp contrast to that on [¹²⁵I]-oxidized low density lipoprotein. These results implicate that CD36-mediated interaction of AGE-modified proteins with adipocytes might play a pathological role in obesity or insulin-resistance.

[学科分类] 生物化学/生物物理

[关键词] Adipocytes;Advanced glycation end product;CD36;Receptor for advanced glycation end product;AGE;advanced glycation end product;AGE-BSA;advanced glycation end product-modified bovine serum albumin;LDL;low density lipoprotein;OxLDL;oxidized LDL;SR-A;scavenger receptor class A