The ‘magic tail’ of G protein‐coupled receptors: an anchorage for functional protein networks

[摘要]

All cell types express a great variety of G protein-coupled receptors (GPCRs) that are coupled to only a limited set of G proteins. This disposition favors cross-talk between transduction pathways. However, GPCRs are organized into functional units. They promote specificity and thus avoid unsuitable cross-talk. New methodologies (mostly yeast two-hybrid screens and proteomics) have been used to discover more than 50 GPCR-associated proteins that are involved in building these units. In addition, these protein networks participate in the trafficking, targeting, signaling, fine-tuning and allosteric regulation of GPCRs. To date, proteins that interact with the GPCR C-terminus are the most abundant and are the focus of this review.

[学科分类] 生物化学/生物物理

[关键词] G protein-coupled receptor;PSD95-disc large-zona occludens domain-containing protein;Ena/VASP homology domain-containing protein;Scaffolding;Targeting;Signaling;AT1;angiotensin II type 1;β1 or β2-AR;β1 or β2 adrenergic receptors;CN-Ras GEF;cyclic nucleotide-Ras guanine nucleotide exchange factor;CREB2;cAMP responsive element binding protein 2;DHPG;dihydroxyphenylglycine;eNOS;endothelial nitric oxide synthase;EVH;Ena/VASP homology;GABA-B;gamma-aminobutyric acid type B;GPCRs;G protein-coupled receptors;IP3;inositol trisphosphate;mGluR;metabotropic glutamate receptor;NHERF;Na+/H+ exchanger regulatory factor (also called EBP ezrin-radixin-moesin binding protein);NMDA;N-methyl-D-aspartate;NR1;NR2;NMDA receptor 1;NMDA receptor 2;PDZ;PSD95-disc large-zona occludens;PICK-1;protein interacting with C kinase 1;PKC;protein kinase C;PSD95;postsynaptic density protein of 95 kDa;PTH;parathyroid hormone;SH;Src homology;Shank;SH3 multiple ankyrin;TcTex-1;t-complex testis-expressed 1