Chondroitin sulfate of appican, the proteoglycan form of amyloid precursor protein, produced by C6 glioma cells interacts with heparin‐binding neuroregulatory factors

[摘要]

Appican produced by rat C6 glioma cells, the chondroitin sulfate (CS) proteoglycan form of the amyloid precursor protein, contains an E disaccharide, –GlcUA-GalNAc(4,6-O-disulfate)–, in its CS chain. In this study, the appican CS chain from rat C6 glioma cells was shown to specifically bind several growth/differentiation factors including midkine (MK) and pleiotrophin (PTN). In contrast, the appican CS from SH-SY5Y neuroblastoma cells contained no E disaccharide and showed no binding to either MK or PTN. These findings indicate that the E motif is essential in the interaction of the appican CS chain with growth/differentiation factors, and suggest that glial appican may mediate the regulation of neuronal cell adhesion and migration and/or neurite outgrowth.

[学科分类] 生物化学/生物物理

[关键词] Alzheimer's disease;Amyloid precursor protein;Appican;Chondroitin sulfate;Heparin-binding growth factor;AD;Alzheimer's disease;Aβ;amyloid β;AβPP;amyloid precursor protein;CS;chondroitin sulfate;GalNAc;N-acetyl-D-galactosamine;GlcUA;D-glucuronic acid;GAG;glycosaminoglycan;HPLC;high performance liquid chromatography;HS;heparan sulfate;ΔHexUA;4-deoxy-α-L-threo-hex-4-enepyranosyluronic acid;MK;midkine;PTN;pleiotrophin;bFGF;basic fibroblast growth factor;HB-EGF;heparin-binding epidermal growth factor-like growth factor;PG;proteoglycan