1,2‐Diacylglycerol hydroperoxides induce the generation and release of superoxide anion from human polymorphonuclear leukocytes

[摘要]

We examined the effect of 1-palmitoyl-2-linoleoylglycerol (PLG), PLG hydroxide (PLG-OH), and PLG hydroperoxide (PLG-OOH) on the release of superoxide anion from human PMNs monitored by the chemiluminescence generated by the superoxide anion-sensitive reagent, 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one (MCLA). PLG-OOH at low micromolar concentrations stimulated human PMNs whereas PLG and PLG-OH did not. 1,3-Dilinoleoylglycerol hydroperoxide, 1-palmitoyl-2-linoleoylphosphatidylcholine hydroperoxide, and linoleic acid hydroperoxide were much less efficient in stimulating human PMNs than PLG-OOH. The PKC inhibitors, chelerythrine chloride and staurosporine, inhibited the stimulation of PMNs. Possible pathophysiological role of 1,2-diacylglycerol hydroperoxides is discussed.

[学科分类] 生物化学/生物物理

[关键词] Diacylglycerol hydroperoxide;Human polymorphonuclear leukocyte;Superoxide anion;2-Methyl-6-(p-methoxyphenyl)-3;7-dihydroimidazo[1;2-a]pyrazin-3-one;Protein kinase C;PKC;protein kinase C;PMA;phorbol 12-myristate 13-acetate;1;2-LLG-OOH;1;2-dilinoleoylglycerol hydroperoxide;1;2-LLG-OOH;1;2-dilinoleoylglycerol hydroxide;1;2-DAG-OOH;1;2-diacylglycerol hydroperoxide;1;2-DAG-OH;1;2-diacylglycerol hydroxide;PMNs;polymorphonuclear leukocyte(s);MCLA;2-methyl-6-(p-methoxyphenyl)-3;7-dihydroimidazo[1;2-a]pyrazin-3-one;PLPC;1-palmitoyl-2-linoleoylphosphatidylcholine;PLPC-OOH;PLPC hydroperoxide;PLPC-OH;PLPC hydroxide;1;3-LLG;1;3-dilinoleoylglycerol;1;3-LLG-OOH;1;3-LLG hydroperoxide;SOD;superoxide dismutase;PLG;1-palmitoyl-2-linoleoylglycerol;PLG-OOH;PLG hydroperoxide;PLG-OH;PLG hydroxide;HBSS;Hanks' balanced salt solution;HPLC;high performance liquid chromatography;MLI;maximal luminescence intensity;cpm;counts/min