Breeding potential of cassava (Manihot esculenta Crantz) in Mozambique
[摘要] English: This study represents the first comprehensive genetic study based on local cultivars of Mozambique. Five cassava (Manihot esculenta Crantz) parents were crossed in a full diallel. The parents and F1 progeny were evaluated for eight agronomical and 17 morphological traits in two consecutive seasons. General combining ability (GCA) was higher than specific combining ability (SCA) for number of roots, root taste, fresh root yield, root pulp hardness and harvest index. Positive heterosis was seen for number of roots, fresh root weight and harvest index. Negative heterosis was present for cassava brown streak virus disease. Harvest index showed high broad sense heritability, which indicated that phenotypic variance was additive. The predictability ratio was close to unity, which confirmed that this trait is highly heritable, and determined by additive gene action. Cassava brown streak root necrosis had SCA effects significantly higher than GCA effects indicating the presence of non-additive genetic effects. A selection index comprised of the most relevant characters was compiled. Amplified fragment length polymorphism (AFLP) analysis showed high genetic similarity among 17 accessions. A total of 425 fragments were produced by eight primer combinations. Mocuba and Nikwaha were the most distinct among the accession and two heterotic groups were suggested. Morphological analysis showed genetic distance ranging from 0.105 to 0.833. The correlation coefficient between AFLP and agronomic clustering was not significant. The AFLP and morphological analysis did not group the accessions according to geographical origin. The principal component analysis provided diversity structure, suggesting the effectiveness of this analysis in genetic diversity studies. The narrow genetic similarity observed, indicates the need to introduce new cassava germplasm.
[发布日期] [发布机构] University of the Free State
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