Biomarker evidence of DNA oxidation in lung cancer patients: association of urinary 8‐hydroxy‐2′‐deoxyguanosine excretion with radiotherapy, chemotherapy, and response to treatment

[摘要]

Ratios of urinary 8-hydroxy-2′-deoxyguanosine to urinary creatinine (8-OHdG/creatinine) have been considered as a good biological indicator of DNA oxidation. Urinary 8-OHdG/creatinine levels of lung cancer patients were evaluated by enzyme-linked immunosorbent assay using a monoclonal antibody N45.1 during radiotherapy and chemotherapy. An increase in urinary 8-OHdG/creatinine was found in non-small-cell carcinoma (non-SCC) patients during the course of radiotherapy. SCC patients showed higher levels of urinary 8-OHdG/creatinine than the controls. Furthermore, SCC patients with complete or partial response to the chemotherapy showed a significant decrease in urinary 8-OHdG/creatinine while patients with no change or progressive disease showed an increase.

[学科分类] 生物化学/生物物理

[关键词] Oxidative DNA damage;Lung cancer;Radiotherapy;Chemotherapy;8-Hydroxy-2′-deoxyguanosine;Monoclonal antibody;8-OHdG;8-hydroxy-2′-deoxyguanosine;SCC;small-cell carcinoma;HPLC;high performance liquid chromatography;ELISA;enzyme-linked immunosorbent assay;Gy;gray;CR;complete response;PR;partial response;NC;no change;PD;progressive disease;ANOVA;an analysis of variance;LSD;least significance difference