Cca3, the mRNA level of which transiently decreases before initiation of DNA synthesis in regenerating rat liver cells

[摘要]

Kinetics of cca1, cca2, cca3 and rat gas1 mRNA levels were compared with those of DNA synthesis level in regenerating rat liver cells after partial hepatectomy. A transient decrease of cca3 mRNA level and an increase of rat gas1 mRNA level were observed before initiation of DNA synthesis, followed by a rapid decrease of rat gas1 mRNA level. By molecular cloning and nucleotide sequencing, cca3 cDNA was found to consist of 4514 nucleotides with a large open reading frame of 3027 nucleotides, encoding a protein of 1009 amino acids with three copies of an ankyrin repeat-like sequence.

[学科分类] 生物化学/生物物理

[关键词] Rat liver regeneration;cca3 cDNA cloning;Ankyrin repeat;aa;amino acid;cca;confluent 3Y1 cell-associated;gas;growth arrest-specific;nt;nucleotide;ORF;open reading frame;PH;partial hepatectomy;RACE;rapid amplification of the cDNA ends;UTR;untranslated DNA region