Preliminary X‐ray crystallographic analysis of tryptophanase from Escherichia coli

[摘要]

Tryptophanase (L-tryptophan indole-lyase) from Escherichia coli has been crystallized from ammonium sulfate solution using a vapor diffusion method. The crystals are tetragonal and belong to space group P4₁2₁2 or its enantiomorph. The cell dimensions of the crystals are a = b = 113.4 Å, and c = 232.2 Å, with two subunits per asymmetric unit. The crystals diffract to at least 3 Å resolution, and are suitable for X-ray structural analysis.

[学科分类] 生物化学/生物物理

[关键词] Tryptophanase;L-Tryptophan indole-lyase: Crystal data;Crystallization;Escherichia coli;PLP;pyridoxal 5′ phosphate