2‐Deoxy‐2‐fluoro‐D‐galactose protein N‐glycosylation

[摘要]

2-Deoxy-2-fluoro-D-galactose (dGalF), added to the medium of primary cultured rat hepatocytes, inhibited N-glycosylation on membrane (gp 120) and secretory glycoproteins (α ₁-macroglobulin) in a concentration dependent manner. Complete inhibition of N-glycosylation was achieved at concentrations of 1 mM and above. At identical concentrations, 2-deoxy-2-fluoro-D-glucose (dGlcF) caused only incomplete inhibition of N-glycosylation. dGalF reduced incorporation of D-[2,6-³H]mannose into lipid-linked oligosaccharides indicating interference with their assembly in the dolichol cycle.

[学科分类] 生物化学/生物物理

[关键词] Inhibition of N-glycosylation;Glycoprotein;Tunicamycin;2-Deoxy-2-fluoro-D-galactose;2-Deoxy-2-fluoro-D-glucose;Hepatocyte;dGalF;2-deoxy-2-fluoro-D-galactose;dGlcF;2-deoxy-2-fluoro-D-glucose;Dol-P;dolichyl monophosphate;Dol-PP;dolichyl pyrophosphate;Fuc;fucose;Gal;galactose;GlcNAc;N-acetyl-glucosamine;NeuAc;N-acetylneuraminic acid;PAGE;polyacrylamide gel electrophoresis;PBS;phosphate buffered saline;SDS;sodium dodecysulfate