Purification and partial characterization of glyoxalase I from a higher plant Brassica juncea

[摘要]

The glyoxalase I was purified from Brassica juncea by affinity chromatography on S-hexyl GSH sepharose 4B, Homogeneity of the protein was confirmed electrophoretically by a silver stained gel. Activity staining on a native starch gel also showed a single band. The effect of glutathione, methylglyoxal, and pH on enzyme kinetics was studied. Magnesium was found to stimulate the enzyme activity.

[学科分类] 生物化学/生物物理

[关键词] Glyoxalase I;Enzyme purification;Methylglyoxal;Glutathione;S-hexyl glutathione;GSH;reduced glutathione;MG;methylglyoxal;PMSF;phenyl methylsulfonylfluoride;PVP;polyvinylpyrroliodone;EGTA;ethylene glycol-bis(β-aminoethyl ether)N;N;N′;N′-tetra acetic acid