High‐velocity mechanical DNA transfer of the chloramphenicolacetyl transferase gene into rodent liver, kidney and mammary gland cells in organ explants and in vivo

[摘要]

Mouse and rat liver, kidney and mammary gland explants were bombarded with high-velocity microprojectiles carrying a chloramphenicolacetyl transferase gene under different promoters (pTAT-cat, p_χ-Cusein-cat, pβ-Cusein-cat). The expression of a CAT gene was revealed in all organ explants 24 h after transfection. The most pronounced expression was found when a TAT-CAT construction was used. In experiments in vivo rat liver was bombarded in situ with microprojectiles carrying a pTAT-cat DNA. A marked activity of the CAT gene was detected 24 h after the bombardment.

[学科分类] 生物化学/生物物理

[关键词] Transfection;High-velocity microprojectile;CAT gene;Rodent cell in vitro and in vivo