Topography and stoichiometry of acidic phosphoproteins in rat liver 60 S ribosomal subunit

[摘要]

In reconstitution experiments of active 60 S subunits from inactive core particles obtained by using dimethyl maleic anhydride (DMMA), we observed that the phosphoproteins P1–P2 were extracted from the subunit by DMMA as a complex with other proteins. This complex was separated by electrophoresis and zonal centrifugation and shown, after ¹²⁵I iodination of its components, to contain L22 and S12 in addition to P1–P2. Results suggest that it contains two copies of P1–P2 for one of L22 and S12.

[学科分类] 生物化学/生物物理