The accumulation of c-fos mRNA in mouse thymocytes was compared when the cells were stimulated by concanavalin A (Con A), the Ca2+ ionophore A23187 or the phorbol ester, TPA, either separately or by combinations of these mitogens. The c-fos response to mitogenic concentrations of Con A could not be attributed either to the rise in [Ca2+]i it induces or to activation of protein kinase C. Thus, although Con A causes the breakdown of phosphatidylinositol 4,5-bisphosphate in these cells, neither of the signals which can be generated by this response was responsible for the activation of the c-fos gene by Con A. This implies that some other unidentified signal generated by Con A activates the c-fos gene.
