Cleavage of specific sites of RNA by designed ribozymes

[摘要]

Two ribozymes were designed for site-specific cleavage of RNA. A UA site in an undecaribonucleotide was cleaved by a ribozyme consisting of two partially paired oligoribonucleotides with chain lengths of 19 and 15. The other ribozyme, which consists of a 19-mer and a 13-mer, recognized a UC sequence at positions 42 and 43 of 5 S rRNA.

[学科分类] 生物化学/生物物理

[关键词] Synthetic oligoribonucleotide;RNA enzyme;Endonucleolytic activity;RNA sequence recognition