Structure determination of the O‐specific polysaccharides from Citrobacter O4‐ and O27‐lipopolysaccharides by methylation analysis and one‐ and two‐dimensional 1 H‐NMR spectroscopy

[摘要]

Using sugar and methylation analyses, and one- and two-dimensional ¹H-NMR spectroscopy at 500 MHz it was established that poly-β-1,2-4-deoxy-D-arabinohexopyranose occurs as O-specific chains of lipopolysaccharides in Citrobacter serotypes O4, O27 and O36. Strong serological cross-reactivity between these serotypes is in full agreement with the chemical identity of their O-specific polysaccharides.

[学科分类] 生物化学/生物物理

[关键词] Structure determination;O-specific polysaccharide;Methylation analysis;1H-NMR;(Citrobacter freundii);COSY;two-dimensional scalar shift-correlated NMR spectrum;D and 2D;one- and two-dimensional;NOE;nuclear Overhauser effect