Studies on interaction of 5 S RNA with ribosomal proteins

[摘要]

Proteins of the large ribosomal subunit of rat liver (TP 60) were immobilized by diffusion transfer onto nitrocellulose after two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Incubation of the TP 60 blots with ³²P-labeled 5 S RNA under defined ionic conditions (300 mM KCl, 20 mM MgCl₂) resulted in specific binding to a limited set of ribosomal proteins consisting of proteins L3, L4, L6, L13/15 and — to a lesser extent — L7 and L19. Under identical conditions, blots with proteins of the small ribosomal subunit (TP 40) did not bind 5 S RNA.

[学科分类] 生物化学/生物物理

[关键词] Ribosome;Ribosomal protein;RNA-protein interaction;Protein blotting;5 S RNA;5 S RNA-binding protein;TP 40;total ribosomal protein from small subunits of rat liver ribosomes;TP 60;total protein from large subunits of rat liver ribosomes;2-D PAGE;two-dimensional polyacrylamide gel electrophoresis