Oxidation‐reduction midpoint potentials of the molybdenum center in spinach NADH:nitrate reductase

[摘要]

Oxidation-reduction midpoint potentials for the molybdenum center in assimilatory NADH:nitrate reductase isolated from spinach (Spinacia oleracea) have been determined at pH 7.0 in the presence of dye mediators using EPR spectroscopy to monitor formation of Mo(V). Values for the Mo(VI)/Mo(V) and Mo(V)/Mo(IV) couples were determined to be −8 and −42 mV, respectively.

[学科分类] 生物化学/生物物理

[关键词] Nitrate reductase;Molybdenum;Redox potential;(Spinacia oleracea);Mops;3-(N-morpholino)propanesulfonic acid;MV.+;methyl viologen radical cation