A new mutation in the gene coding for the herbicide‐binding protein in Chlamydomonas

[摘要]

Sequence analysis of herbicide-resistant Chlamydomonas mutants revealed a new mutation in the psb A gene coding for the herbicide-binding (D1) protein. A point mutation at codon 251 leading to an amino acid substitution from alanine in wild-type cells to valine in the mutant was identified by sequencing total cellular RNA. The folding pattern of D1 predicts Ala 251 to be part of the Qb-binding niche.

[学科分类] 生物化学/生物物理

[关键词] Herbicide-binding protein;Herbicide resistance;Point mutation;Amino acid substitution;RNA sequencing;(Chlamydomonas)