Pertussis toxin inhibits the angiotensin II and serotonin‐induced rise of free cytoplasmic calcium in cultured smooth muscle cells from rat aorta

[摘要]

Angiotensin II, serotonin and K⁺-depolarization cause an increase in free cytoplasmic Ca²⁺ in cultured smooth muscle cells. The involvement of a guanine nucleotide-binding protein has been investigated by using pertussis toxin. When smooth muscle cells were pretreated with pertussis toxin angiotensin II and serotonin-induced rise of cytosolic Ca²⁺ was found to be significantly reduced whereas the Ca²⁺ influx mediated by K⁺-depolarization remained unchanged. These results suggest the participation of a guanine nucleotide-binding protein in the receptor-mediated rise of intracellular Ca²⁺.

[学科分类] 生物化学/生物物理

[关键词] Angiotensin II;Serotonin;intracellular Ca2+;Pertussis toxin;(Smooth muscle cell)