Down‐regulation of phorbol diester binding to NG115‐401L neuronal cells is dependent on structure, concentration and time

[摘要]

The down-regulation of [³H]PDBu binding to a neural cell line, NG115-401L, has been examined in response to two biologically active phorbol diesters, PDBu and PMA. Chronic treatment with PDBu or PMA causes a concentration- and time-dependent loss of specific [³H]PDBu binding. The action of PMA is biphasic with respect to both concentration and time dependence.

[学科分类] 生物化学/生物物理

[关键词] Phorbol ester;Protein kinase C;Phorbol diester binding;Down-regulation;(Neuronal cell line);PDBu;phorbol 12;13-dibutyrate;PMA;phorbol 12-myristate 13-acetate;PKC;protein kinase C;DMEM;Dulbecco's modified Eagle's medium;FCS;fetal calf serum;BSA;bovine serum albumin;HGB;Hank's saline supplemented with 8 mg/ml glucose and 2 mg/ml BSA