The enhanced 32P labeling of CDP‐diacylglycerol in c‐myc gene expressed human kidney cancer cells

[摘要]

Correlation between c-myc gene expression and phosphatidylinositol (PI) metabolism was studied using seven human kidney cancer cell lines. We found that the exceptional incorporation of ³²P into CDP-diacyl-glycerol (CDP-DG) was observed in only two cell lines, in which c-myc expression was increased among seven cell lines tested. In these two cell lines, PI was also labeled. The other five cell lines indicated the accumulation of radioactive PI, whereas CDP- DG was unlabeled.

[学科分类] 生物化学/生物物理

[关键词] c-myc gene;Phosphatidylinositol metabolism;CDP-diacylglycerol;(Kidney cancer cell);PI;phosphatidylinositol;PIP;phosphatidylinositol 4-phosphate;PIP2;phosphatidylinositol 4;5-bisphosphate;PG;phosphatidylglycerol;TLC;thin-layer chromatography;HPTLC;high-performance TLC