Luteinizing hormone‐releasing hormone (LHRH)‐induced arachidonic acid release in rat granulosa cells Role of calcium and protein kinase C

[摘要]

In rat granulosa cells prelabeled with [³H]arachidonic acid, addition of LHRH, A23187 or 12-0-tetradecanoyl phorbol-13-acetate (TPA) enhanced the release of [³H]arachidonic acid into the culture medium. The effect of A23187 was significant as early as 5 min and the lowest effective dose was 5 × 10⁻⁸/M. On the other hand, TPA was effective only at dosages greater than 10⁻⁶M. These results suggest that the stimulatory effect of LHRH on arachidonic acid release is coupled more tightly to a Ca²⁺-dependent rather than a protein kinase C-mediated pathway.

[学科分类] 生物化学/生物物理

[关键词] Luteinizing hormone-releasing hormone;Arachidonic acid;Phorbol ester;Ca2+;Protein kinase C;(Ovary)