Dimethylallylpyrophosphate:3,9‐dihydroxypterocarpan 10‐dimethylallyl transferase from Phaseolus vulgaris Identification of the reaction product and properties of the enzyme

[摘要]

A microsomal fraction of elicitor-challenged bean (Phaseolus vulgaris) cell suspension cultures catalyses the prenylation of 3,9-dihydroxypterocarpan (DHP) to 3,9-dihydroxy-10-dimethylallylpterocarpan (phaseollidin) with dimethylallylpyrophosphate (DMAPP) as the prenyl donor. K _m values for DMAPP and DHP are in the range 1–3 μM. Strong product inhibition with phaseollidin and phaseollin is observed.

[学科分类] 生物化学/生物物理

[关键词] Prenyltransferase;Phaseollidin;Cell culture;(Phaseolus vulgaris)