[摘要] Preformed phosphatidylethanolamine-containing liposomes were thiolated with 2-iminothiolane (Traut's reagent) and subsequently activated by mixed disulfide formation with 5,5′-dithiobis(2-nitrobenzoic acid). Up to 65% of amino groups of the outer liposomal lamella, corresponding to 230 SH-groups per vesicle, were modified. Covalent attachment of thiolated α-chymotrypsin to these thiol-liposomes via S-S bridges yielded a protein/lipid ratio of 3.6 × 10−4 mol enzyme/mol lipid.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物化学/生物物理
[关键词] Thiol-liposome;2-Iminothiolane;Membrane thiolation;α Immobilization;α-Chymotrypsin;DTNBS;5;5′-dithiobis(2-nitrobenzoic acid);SPDP;N-hydroxysuccinimidyl-3-(2-pyridyldithio)propionate;PDP-;N-3-(2-pyridyldithio)propionyl-;PC;phosphatidylcholine;PE;phosphatidylethanolamine;DPPE;dipalmitoylphosphatidylethanolamine;ATEE;acetyltyrosine ethyl ester;α-CT;α-chymotrypsin;DTE;dithiothreitol;TNBS;2;4;6-trinitrobenzene sulfonic acid;SUV;small unilamellar vesicle [时效性]
