Phosphoglycerate kinase from the extreme thermophile Thermus thermophilus Crystallization and preliminary X‐ray data

[摘要]

The glycolytic enzyme phosphoglycerate kinase has been prepared from the extreme thermophile Thermus thermophilus. In contrast to its eukaryote equivalents (yeast and horse muscle) this prokaryotic enzyme crystallizes from ammonium sulphate in the presence of nucleotide substrates. These crystals are of a form and size well suited to high resolution X-ray diffraction study using rotation camera techniques.

[学科分类] 生物化学/生物物理

[关键词] Glycolytic enzyme;Crystal growth;Nucleotide substrate;X-ray diffraction;(Thermus thermophilus)