[摘要] Exchange of bound alkali light chains on myosin by free alkali light chains is described. It was found that the yield of hybrid obtained was dependent on the incubation time in 4.7 M NH4Cl at pH 9.5. 60% recovery of S1 (A1) from S1(A2) was obtained using only a 2-fold molar excess of A1 over S1(A2).
[发布日期] [发布机构]
[效力级别] [学科分类] 生物化学/生物物理
[关键词] Actin;Myosin;Myosin subfragment 1;Alkali light chain;Hybridization;S1;myosin subfragment 1;S1(A1);S1(A2);rabbit muscle myosin subfragment 1 containing either the alkali 1 light chain (A1) or the alkali 2 light chain (A2);Bicine;N;N-bis(2-hydroxyethyl)glycine;resin SE-53;sulphoxyethyl cellulose [时效性]
