Synthesis and turnover rates of four rat liver mitochondrial RNA species

[摘要]

The synthesis and turnover rates of the two 12 S and 16 S mt rRNAs and of the mt mRNAs for subunits I and III of cytochrome oxidase have been determined by measuring the kinetics of incorporation of [³H]uridine in the mtRNA of rat hepatocytes. All the RNA species examined have approximately the same turnover (t ~ 100 min) and therefore the rate of synthesis, which is about 10-times higher for the rRNAs, seems to be the factor responsible for the different mt rRNA and mRNA steady-state levels.

[学科分类] 生物化学/生物物理

[关键词] mtRNA synthesis rate;Turnover rate;Transcription regulation;(Rat hepatocyte);mt;mitochondrial;bp;base pair(s);COI;COIII;subunit I and III of cytochrome oxidase