Formation of lipoxin A by granulocytes from eosinophilic donors

[摘要]

The formation of arachidonic acid-derived lipoxygenase products was examined with human granulocytes obtained from eosinophilic donors. These eosinophil-enriched leukocyte populations, challenged in vitro with the ionophore of divalent cations A23187, transformed both exogenous and endogenous sources of arachidonic acid to several lipoxygenase-derived products, including 5(S),6(R),15(S)-trihydroxy-7,9, 13-trans-11-cis-eicosatetraenoic acid (lipoxin A). Lipoxin A was detected and characterized by high-pressure liquid chromatography (HPLC), ultraviolet absorbance, and gas-liquid chromatography-mass spectroscopy. Neither lipoxin B nor 6(S)-LXA was consistently detected in extracts from these incubations. The amounts of lipoxin A formed were proportional to the percentage of eosinophils present in the suspension. The results indicate that granulocytes from eosinophilic donors can generate lipoxin A.

[学科分类] 生物化学/生物物理

[关键词] Lipoxygenase product;Eosinophil;Arachidonic acid;Leukocyte;(Human);RP-HPLC;reverse-phase high-pressure liquid chromatography;GC;gas-liquid chromatography;MS;mass spectrometry;Me3Si;trimethylsilyl;LX;lipoxin;lipoxin A (LXA);5(S);6(R);15(S) -trihydroxy-7;9;13-trans-11-cis-eicosatetraenoic acid;6(S)-LXA;5(S);6(S);15(S)-trihydroxy-7;9;13-trans-11 -cis-eicosatetraenoic acid;lipoxin B (LXB);5(S);14(R);15(S) -trihydroxy-6;10;12-trans-8-cis-eicosatetraenoic acid;15 -HETE;15(S)-hydroxy-5;8;11-cis-13-trans -eicosatetraenoic acid;HES;hypereosinophilic syndrome;5;15 -DHETE;5(S);15(S)-dihydroxy-6;13-trans-8;11-cis -eicosatetraenoic acid