Mg2+‐ATP induces filament growth from retinal rod outer segments with disrupted plasma membranes

[摘要]

Mg²⁺-ATP produces a large decrease in near-IR light scattering when added to suspensions of rod outer segments (ROS) when the plasma membranes have been disrupted by a gentle dialysis procedure. When this process is studied by light microscopy with video-enhanced image contrast, the Mg²⁺-ATP-dependent signal is seen to be associated with the formation of filaments which extend only from those ROS lacking plasma membranes. Both the IR light scattering signal and filament growth are inhibited by vanadate and DCCD but not by colchicine, colcemid or cytochalasins.

[学科分类] 生物化学/生物物理

[关键词] Mg2+-ATP;Cytoskeleton;Filament growth;Rod outer segment;Light scattering;Vision;AMP-PNP;adenylyl imidodiphosphate;DCCD;N;N'-dicyclohexylcarbodiimide;IR;infrared;ROS;rod outer segments