Resonance Raman study of intermediates of the halorhodopsin photocycle

[摘要]

The resonance Raman (RR) study of the retinal protein halorhodopsin (HR₅₇₈) was extended to two of its photoproducts: HR and HR^L ₄₁₀ RR spectra of both species were recorded in H₂O and D₂O and compared with the RR spectra of the intermediates L₅₅₀ and M₄₁₂ from the bacteriorhodopsin photocycle. HR₅₂₀ was found to be a protonated Schiff base in the 13-cis configuration and HR^L ₄₁₀ a deprotonated Schiff base in the 13-cis configuration.

[学科分类] 生物化学/生物物理

[关键词] Resonance Raman spectroscopy;Photocycle;Light-driven chloride pump;Retinal isomerization;Retinal protein