Amino acid sequence surrounding the lipoic acid cofactor of bovine kidney 2‐oxoglutarate dehydrogenase complex

[摘要]

The 2-oxoglutarate dehydrogenase complex was succinylated using 2-oxo[5-¹⁴C]glutarate in the presence of N-ethylmaleimide to label the lipoic acid cofactor of the transuccinylase (E2) component. Following peptic digestion, ¹⁴C-lipoate-containing peptides were purified and subjected to automated Edman degradation and amino acid analysis. The amino acid sequence surrounding the lipoyllysine residue is reported.

[学科分类] 生物化学/生物物理

[关键词] 2-Oxoglutarate dehydrogenase complex;Lipoic acid;Succinylation;Primary structure;OGDC;2-oxoglutarate dehydrogenase complex;PDC;pyruvate dehydrogenase complex