Arginine‐vasopressin stimulates the formation of phosphatidic acid in rat Leydig cells

[摘要]

Arginine-vasopressin (AVP) stimulated the formation of labelled phosphatidic acid (PA) in [¹⁴C]arachidonic acid-prelabelled rat Leydig cells. After addition of 10^t̄6M AVP [¹⁴C]arachidonoylphosphatidic acid reached a maximum within 2 min. The increase was dose-dependent (10⁻¹¹−10⁻⁶ M). No change in labelling of other phospholipids and diacylglycerol could be detected. The V₁ antagonist dPTyr(Me)AVP inhibited in a dose-dependent manner the AVP-stimulated accumulation of PA. The V₂ agonist dPVDAVP was without effect. The present results suggest that AVP binds to V₁ receptors in rat Leydig cells resulting in stimulation of PA turnover. We suggest that the AVP-stimulated PA formation is an indication of phosphoinositide turnover.

[学科分类] 生物化学/生物物理

[关键词] Arginine-vasopressin;Phosphatidic acid;V1 receptor;Arachidonic acid;Diacylglycerol;(Leydig cell);AVP;arginine-vasopressin;dPTyr(Me)AVP;[1-deaminopenicillamine;(O-methyl)Tyr2;Arg8]vasopressin;dPVDAVP;[1-deaminopenicillamine;Val4;Arg8]vasopressin;PI;phosphatidylinositol;PIP + P1P22;phosphatidylinositol 4-phosphate + phosphatidylinositol 4;5-bisphosphate;DG;diacylglycerol;PA;phosphatidic acid