BliI, a restriction endonuclease from Bacillus licheniformis

[摘要]

From Bacillus licheniformis a site-specific restriction endonuclease, named BliI, has been purified and characterized. BliI was able to digest λ DNA at pH 9.1 over a wide temperature range (25–65°C). Digestion of λ and (ϕX174 DNAs with BliI produced banding patterns identical to those seen with HaeIII. Therefore, BliI and HaeIII endonculeases are isoschizomers.

[学科分类] 生物化学/生物物理

[关键词] Restriction enzyme;HaeIII isoschizomer;(Bacillus licheniformis)