Properties of 2′,3′‐dideoxy‐2′,3′‐dehydrothymidine 5′‐triphosphate in terminating DNA synthesis catalyzed by several different DNA polymerases

[摘要]

2′-3′-Dideoxy-2′,3′-dehydrothymidine 5′-triphosphate (dddTTP) shows termination substrate properties in the DNA synthesis catalyzed by E. coli DNA polymerase I KF, rat liver DNA polymerase β, reverse transcriptases of avian myeloblastosis virus and Raus sarcoma virus and calf thymus terminal deoxynucleotidyl transferase. This implies that the mononucleotide residue of dddTTP incorporates into 3′-termini of newly synthesized DNA chains. However, dddTTP has no influence on the DNA synthesis catalyzed by calf thymus DNA polymerase α. In the case of some DNA polymerases dddTTP was one order of magnitude more effective in comparison with the other known termination substrates.

[学科分类] 生物化学/生物物理

[关键词] DNA polymerase;2′;3′-Dideoxy-2′;3′-dehydrothymidine 5′-triphosphate;Termination substrate;dNTP;2′-deoxynucleoside 5′-triphosphates with natural bases;dNTP(3′-F);dNTP(3′-NH2);dNTP(3′-N3) and dNTP(3′-NHacet);5′-triphosphates of 3′-fluoro-;3′-amino-;3′-azido- and 3′-acetamido-2′;3′-dideoxynucleosides;ddNTP;2′;3′-dideoxynucleoside 5′-triphosphates;dNTP(2′-OCH3);3′-O-methyl-dNTP;araNTP(3′-N3) and araNTP(3′-NH2);5′-triphosphates of 3′-azido- and 3′-amino-3′-deoxyarabinonucleosides