Expression of ricin A chain in Escherichia coli

[摘要]

DNA encoding ricin A chain was derived from preproricin cDNA and ligated into the expression vector pDS5/3. Transcription is controlled from the coliphage promoter P_N25 fused with the lac operator of E.coli. When induced, E.coli 71.18 cells transformed with the recombinant plasmid express ricin A chain which is soluble and has full biological activity.

[学科分类] 生物化学/生物物理

[关键词] Ricin A chain;Expression;Immunotoxin;(E.coli)