Clathrin β‐light chain of rat liver coated vesicles is phosphorylated in vitro and in vivo

[摘要]

Clathrin β-light chain of rat liver coated vesicles is phosphorylated in vitro in the presence of poly(L-lysine) by an endogenous protein kinase which appears to be similar to casein kinase II. Clathrin β-light chain is also phosphorylated in vivo. After injection of [³²P]phosphate into rats and preparation of purified coated vesicles in the presence of phosphatase inhibitors, electrophoretic analysis showed the presence of several labeled polypeptides including clathrin β-light chain. A polypeptide of 50 kDa, which may correspond to the major polypeptide phosphorylated in vitro of coated vesicles, is also labeled in vivo.

[学科分类] 生物化学/生物物理

[关键词] Coated vesicle;Poly(L-lysine);Clathrin β-light chain;Casein kinase II;Protein phosphorylation;Mes;(2-(N-morpholino)ethanesulfonic acid;PMSF;phenylmethylsulfonylfluoride;PAGE;polyacrylamide gel electrophoresis;PNPP;p-nitrophenyl phosphate;disodium salt;NEM;N-ethylmaleimide;PCA;perchloric acid