Low temperature spectrophotometry on the photoreaction cycle of sensory rhodopsin

[摘要]

The photoreaction of sensory rhodopsin (sR) was studied by using low temperature spectrophotometry. Upon irradiation of sR₅₉₀ at temperatures ranging from −30 to −60°C, sR₃₇₃, a photointermediate was produced without any preceding photoproducts. The formation of sR₃₇₃ was temperature-dependent, and an Arrhenius plot gave an activation energy of 15.7 kcal/mol, suggesting that there might be a potential barrier in the excited state of sR₅₉₀.

[学科分类] 生物化学/生物物理

[关键词] Sensory rhodopsin;Photointermediate;Photoreaction;Potential barrier;Activation energy;Low-temperature spectrophotometry;(Halobacterium halobium);Mops;3-(N-morpholino)propanesulfonic acid