Functional expression of cloned cDNA encoding the α‐subunit of adenylate cyclase‐stimulating G‐protein

[摘要]

Cloned cDNA encoding the α-subunit of the adenylate cyclase-stimulating G-protein (G_s), carried by a simian virus 40 vector, has been introduced into the cyc⁻ variant of S49 lymphoma cells by electroporation. In contrast to untransfected cys⁻ cells, clones transformed with the cDNA exhibit an increase in intracellular cyclic AMP concentration in response to a β-adrenergic agonist.

[学科分类] 生物化学/生物物理

[关键词] Adenylate cyclase-stimulating G-protein;cDNA;β-Adrenergic agonist;cyclic AMP;Cholera toxin dependence;ADP-ribosylation;(cyc− S49 lymphoma cell);G-protein;guanine nucleotide-binding regulatory protein;Gs;adenylate cyclase-stimulating G-protein;Gi;adenylate cyclase-inhibiting G-protein;SV40;simian virus 40;DMEM;Dulbecco's modified Eagle's medium;IBMX;3-isobutyl-1-methylxanthine