Regional distribution of leukotriene and mono‐hydroxyeicosatetraenoic acid production in the rat brain Highest leukotriene C4 formation in the hypothalamus

[摘要]

The regional distribution of ionophore A23187-induced synthesis of leukotrienes and mono-hydroxyeicosatetraenoic acids in the rat brain in vitro was investigated. Pronounced differences in leukotriene C₄ formation were observed, with the highest synthetic capacity in the hypothalamus. The formation of leukotriene C₄ was about 12-times higher in the hypothalamus as compared to the cerebellum. This finding is in agreement with a possible neuroendocrine role for leukotriene C₄. In contrast, the activity of leukotriene B₄ synthesis was widely distributed without pronounced regional differences in the rat brain. Formation of 5-, 9-, 11-, 12- and 15-monohydroxyeicosatetraenoic acid was detected in all regions. The major lipoxygenase product in the hypothalamus and thalamus was 5-hydroxyeicosatetraenoic acid, while other monohydroxyeicosatetraenoic acids predominated in the remaining regions tested.

[学科分类] 生物化学/生物物理

[关键词] Hypothalamus;Lipoxygenase product;Leukotriene synthesis;(Rat brain);5-HETE;5-hydroxy-6;8;11;14-eicosatetraenoic acid;9-HETE;9-hydroxy-5;7;11;14-eicosatetraenoic acid;11-HETE;11-hydroxy-5;8;12;14-eicosatetraenoic acid;12-HETE;12-hydroxy-6;8;10;14-eicosatetraenoic acid;15-HETE;15-hydroxy-5;8;11;13-eicosatetraenoic acid.