Evidence of an α2‐macroglobulin‐like molecule in plasma of Salamandra salamandra Structural and functional similarity with human α2‐macroglobulin

[摘要]

A high-M_r (M _r 750 000) α₁ -macroglobulin, obtained from Salamandra salamandra, is described. Salamander α₁-macroglobulin is composed of two monomers of equal M _r, which are composed of two polypeptide chains, each of M _r 180 000, linked by disulfide bonds. The molecular parameters of this protein, its binding to trypsin and inactivation by methylamine suggest that salamander α₁-macroglobulin is closely related to human α₂-macroglobulin and to other related proteins described in the animal kingdom.

[学科分类] 生物化学/生物物理

[关键词] α1-Macroglobulin;α2-Macroglobulin;Trypsin protecting activity;Antiprotease;(Salamander;Urodele);α2M;α2-macroglobulin;α1M;α1-macroglobulin;STI;soybean trypsin inhibitor;BSA;bovine serum albumin;TAME;N-α-p-toluenesulfonyl-L-arginine methyl ester;2ME;2-mercaptoethanol;PAGE;polyacrylamide gel electrophoresis