Purification and properties of an N‐acetylgalactosamine specific lectin from the plant pathogenic fungus Rhizoctonia solani

[摘要]

A lectin was isolated from Rhizoctonia solani mycelium by affinity chromatography on gum arabic-Sepharose. It is a dimeric protein composed of 2 identical subunits of 13 kDa with high contents of asparagine/ aspartic acid, valine, glycine, glutamine/glutamic acid and lysine. The R. solani agglutinin (RSA) exhibits specificity towards N-acetylgalactosamine, and preferentially agglutinates human type A over type B and type O erythrocytes.

[学科分类] 生物化学/生物物理

[关键词] Lectin;Purification;N-Acetylgalactosamine;Gum arabic;(Rhizoctonia solani);PBS;phosphate-buffered saline;RSA;Rhizoctonia solani agglutinin;SDS-PAGE;SDS-polyacrylamide gel electrophoresis