The pH sensitivity of benzodiazepine binding suggests that a histidine residue may be present in, or close to the benzodiazepine binding site. This was confirmed by the selective modification of histidine residues using diethyl pyrocarbonate which was found to block both benzodiazepine and β-carboline binding. In order to assess whether this histidine residue is located in or adjacent to the benzodiazepine and β-carboline binding sites, experiments were performed using either benzodiazepine or β-carboline to protect against diethyl pyrocarbonate treatment. It was found that benzodiazepine agonists, but not propyl β-carboline protect the benzodiazepine binding sites from diethyl pyrocarbonate modification.
