Purification from human plasma of a tetrapeptide that potentiates insulin‐like growth factor‐I activity in chick embryo cartilage
[摘要] Human plasma has been shown to contain a low molecular weight factor that potentiates human IGF-I stimulation of glycosaminoglycan synthesis in chick embryo cartilage. The peptide was purified and characterized by Edman degradation and electrospray mass spectrometry. The primary structure determined was: Trp-Gly-His-Glu. A homologous synthetic peptide similarly promoted matrix biosynthesis in cartilage exposed to IGF-1.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物化学/生物物理
[关键词] Glycosaminoglycan synthesis;Insulin-like growth factor I;Peptide purification;Wound repair;Human plasma;Chick embryo chondrocyte;IGF;insulin-like growth factor;Da;Daltons;rh;recombinant human;MW;molecular weight;RP HPLC;reverse phase high-performance liquid chromatography;Boc;t-Butyloxycarbonyl;BOP;Castro's reagent;TFA;trifluoroacetic acid [时效性]
