Low frequency MFs increased inositol 1,4,5‐trisphosphate levels in the Jurkat cell line

[摘要]

We have earlier reported that when a weak 50 Hz MF (magnetic field) was applied, the leukemic T-cell line Jurkat responded with intracellular calcium oscillations [Lindström, et al., J. Cell Physiol., 156 (1993) 395–398]. The result suggested that the MF interfered with the signal transduction, although neither target molecules nor molecular mechanisms are at present known. In this study we found that application of a MF to Jurkat cells resulted in significant increase of inositol 1,4,5-trisphosphate (IP₃) levels. Chelation of intracellular calcium ions by BAPTA/AM, did not block the increase of IP₃ induced by MF. This result implied that MF-induced Ca²⁺ oscillations were not due to direct stimulation of the Ca²⁺-dependent phospholipase C-γ1 (PLC-γ1).

[学科分类] 生物化学/生物物理

[关键词] Inositol 1;4;5-trisphosphate;Jurkat T-cell line;Ionomycin;BAPTA/AM;Low frequency magnetic field (50 Hz);BAPTA;1;2-bis(2-aminophenoxy)ethane-N;N;N′;N′-tetraacetate;TCR;T cell receptor;MF;magnetic field;[Ca2+]i;intracellular calcium concentration;IP3;inositol 1;4;5-trisphosphate;PLC-γ1;phospholipase C-γ1;MAb;monoclonal antibody