Squamous cell carcinoma antigen is a potent inhibitor of cysteine proteinase cathepsin L
[摘要] A squamous cell carcinoma antigen (SSCA), which is a member of the serpin family of proteinase inhibitors, was purified from sera of cancer patients. It did not inhibit serine proteinases. However, it non-competitively inhibited human cathepsin L with a K i of 0.064 nM, but not cathepsins B and H among cysteine proteinases. These results indicated that SCCA is a non-functional serpin that inhibits cathepsin L in cancer cells.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物化学/生物物理
[关键词] Squamous cell carcinoma antigen (SCCA);Serpin;Proteinase inhibitor;Cathepsin L;SCCA;squamous cell carcinoma antigen;FPLC;fast protein liquid chromatography;HPLC;high performance liquid chromatography;Boc;t-butyloxycarbonyl;Suc;succinyl;Z;carbobenzoxy;Suc(OMe);N-methoxysuccinyl;MCA;4-methylcoumaryl-7-amide;DTT;dithiothreitol;SDS;sodium dodecyl sulfate [时效性]
