Insulin and insulin‐like growth factor I stimulate expression of the primary response gene cMG1/TIS11 b by a wortmannin‐sensitive pathway in RIE‐1 cells

[摘要]

The addition of insulin or insulin-like growth factor I (IGF-I) to RIE-1 cells increased the expression of the primary response gene cMG1; dose-response analysis suggested that this effect was mediated largely through type 1 IGF receptors. Insulin/IGF-I did not affect the expression of the cMG1-related genes TIS11 and TIS11d, whereas epidermal growth factor, angiotensin II or 12-O-tetradecanoyl expression of all three genes. Incubation with wortmannin (WM) prevented the insulin/IGH-I-induced elevation of cMG1 mRNA, but not that induced by the other mitogens or the stimulation of mitogen-activated protein kinase by insulin. We conclude that WM-sensitive phosphatidylinositol 3-kinase may be involved in the specific stimulation of cMG1 expression by insulin/IGF-I.

[学科分类] 生物化学/生物物理

[关键词] Insulin;Primary response gene;Phosphatidylinositol 3-kinase;Mitogen-activated protein kinase;RIE-1 epithelial cell;AII;angiotensin II;EGF;epidermal growth factor;GAPDH;glyceraldehyde phosphate dehydrogenase;IBMX;3-isobutyl-1-methylxanthine;IGF;insulin-like growth factor;MAP kinase;mitogen-activated protein kinase;PI3K;phosphatidylinositol 3-kinase;PI-3;4;5-P3;phosphatidylinositol 3;4;5-trisphosphate;PKC;protein kinase C;TPA;12-O-tetradecanoyl phorbol-13-acetate;WM;wortmannin