Induction of the antioxidant stress proteins heme oxygenase‐1 and MSP23 by stress agents and oxidised LDL in cultured vascular smooth muscle cells

[摘要]

Enhanced expression of the antioxidant stress proteins heme oxygenase-1 (HO-1) and macrophage stress protein (MSP23) by oxidative stress agents and oxidatively modified low density lipoproteins (LDL) was investigated in cultured porcine aortic smooth muscle cells. Treatment of smooth muscle cells with glucose oxidase, CdCl₂ or diethylmaleate resulted in a time-dependent (6–48 h) induction of HO-1 and MSP23 expression. Exposure of cells to 100 μg protein/ml highly oxidised LDL increased the expression of HO-1 and MSP23 within 24 h, and the induction was dependent on the degree of LDL oxidation. The induction of HO-1 and MSP23 may thus play an important cytoprotective role against oxidative stress in atherogenesis.

[学科分类] 生物化学/生物物理

[关键词] Smooth muscle cell;Heme oxygenase-1;MSP23;Oxidative stress protein;Oxidized low density lipoprotein;Atherosclerosis;HO-1;heme oxygenase-1;MSP23;macrophage stress protein;SMC;smooth muscle cells;ROS;reactive oxygen species;nLDL;native low density lipoprotein;moxLDL;mildly oxidised LDL;oxLDL;highly oxidised LDL;DEM;diethylmaleate;GO;glucose oxidase